β-Lactoglobulin Gene Polymorphism of Indigenous Nigerian Goat Breeds Using Polymerase Chain Reaction- Restriction Fragment Length Polymorphism

Authors

  • A. E Ezewudo Federal University of Technology, P.M.B 65, Minna, Niger State, Nigeria.
  • R. G. Abubakar Federal University of Technology, P.M.B 65, Minna, Niger State, Nigeria.
  • S. S. A. Egena Federal University of Technology, P.M.B 65, Minna, Niger State, Nigeria.
  • J. O. Alabi Federal University of Technology, P.M.B 65, Minna, Niger State, Nigeria.

Keywords:

polymorphism, goats, gene locus, PCR-RFLP, β-Lactoglobulin

Abstract

An investigation of the genetic polymorphism at the β-Lactoglobulin (β-LG) gene of Nigerian indigenous goat breeds (West
African Dwarf, Sahel and Red Sokoto) was carried out using Polymerase Chain Reaction-Restriction Fragment Length
Polymorphism (PCR-RFLP) method. Restriction endonucleases (Rsal) were used in the study to cut the extracted DNA into
fragments. The results provided evidence that the β-LG gene locus of West African Dwarf, Red Sokoto and Sahel goats is
monomorphic with only one genetic variant (allele A) with a gene frequency of 1.00. The amplified DNA products were observed
at 120 bp. The restriction digestion with sal revealed just one genotype at the β-LG locus. In conclusion, the results revealed the
absence of polymorphism at the β-LG ocus of the goats.

Author Biographies

A. E Ezewudo, Federal University of Technology, P.M.B 65, Minna, Niger State, Nigeria.

Department of Animal Production

R. G. Abubakar, Federal University of Technology, P.M.B 65, Minna, Niger State, Nigeria.

Department of Animal Production

S. S. A. Egena, Federal University of Technology, P.M.B 65, Minna, Niger State, Nigeria.

Department of Animal Production

J. O. Alabi, Federal University of Technology, P.M.B 65, Minna, Niger State, Nigeria.

Department of Animal Production

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Published

2024-07-09

How to Cite

Ezewudo, A. E., Abubakar, R. G., Egena, S. S. A., & Alabi, J. O. (2024). β-Lactoglobulin Gene Polymorphism of Indigenous Nigerian Goat Breeds Using Polymerase Chain Reaction- Restriction Fragment Length Polymorphism. Nigerian Journal of Animal Production, 7–9. Retrieved from https://njap.org.ng/index.php/njap/article/view/4074

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