β-Lactoglobulin Gene Polymorphism of Indigenous Nigerian Goat Breeds Using Polymerase Chain Reaction- Restriction Fragment Length Polymorphism
DOI:
https://doi.org/10.51791/njap.vi.4074Keywords:
polymorphism, goats, gene locus, PCR-RFLP, β-LactoglobulinAbstract
An investigation of the genetic polymorphism at the β-Lactoglobulin (β-LG) gene of Nigerian indigenous goat breeds (West
African Dwarf, Sahel and Red Sokoto) was carried out using Polymerase Chain Reaction-Restriction Fragment Length
Polymorphism (PCR-RFLP) method. Restriction endonucleases (Rsal) were used in the study to cut the extracted DNA into
fragments. The results provided evidence that the β-LG gene locus of West African Dwarf, Red Sokoto and Sahel goats is
monomorphic with only one genetic variant (allele A) with a gene frequency of 1.00. The amplified DNA products were observed
at 120 bp. The restriction digestion with sal revealed just one genotype at the β-LG locus. In conclusion, the results revealed the
absence of polymorphism at the β-LG ocus of the goats.
