Spermatozoa Quality in Refrigerated Normal Saline Diluted Caprine Semen

Authors

  • U. D. G. Ihejirika University of Ibadan, Ibadan, Oyo State, Nigeria.
  • E. O. Ewuola University of Ibadan, Ibadan, Oyo State, Nigeria.

Keywords:

Semen qualities, normal saline, goat semen extension

Abstract

This study aimed at evaluating the ability of normal saline diluent to sustain spermatozoa viability over a period of time at refrigerated temperature (4-6°C). Semen was collected from 17 West African Dwarf (WAD) bucks, pooled and divided into 5 portions. Four portions were diluted with normal saline at ratio 1:1 (T2), 1:2 (T3), 1:3 (T4) and 1:4 (T5), while unextended portion (T1) served as a control in a completely randomised design. Spermatozoa characteristics were examined 2 hours interval at 4-6°C and Data were analysed using descriptive statistics and ANOVA. Results showed that at 0 hour, spermatozoa motility was significantly (p<0.05) higher in T1, T3 and T4 than T5, although similar to T2. At 6 hours, T2, T4 and T5 were significantly (p<0.05) higher than T1, but similar to T3. At 8 hours, the motility in T1 was significantly (p<0.05) reduced than in other treatments. Spermatozoa livability and acrosome integrity at 0, 2, 4 and 10 hours were not significantly different among the treatments. Plasma membrane integrity at 4 and 8 hours, T3 was significantly (p<0.05) lower than T2, however it was similar to T1, T4 and T5. At 6 hours, plasma membrane integrity of sperm cells in T1 was significantly (p<0.05) lower than T2, T3, T4 and T5. Percentage normal sperm cells at 0 and 6 hours was significantly (p<0.05) lower in T4 than T1, T2 and T3, but similar to T5 and T3 at 6th hour. This suggests that quality of the sperm cells was sustained up to 8 hours in normal saline diluted semen when refrigerated.

Author Biographies

U. D. G. Ihejirika, University of Ibadan, Ibadan, Oyo State, Nigeria.

Department of Animal Science, Faculty of Agriculture  

E. O. Ewuola, University of Ibadan, Ibadan, Oyo State, Nigeria.

Department of Animal Science, Faculty of Agriculture 

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Published

2024-07-09

How to Cite

Ihejirika, U. D. G., & Ewuola, E. O. (2024). Spermatozoa Quality in Refrigerated Normal Saline Diluted Caprine Semen. Nigerian Journal of Animal Production, 326–329. Retrieved from https://njap.org.ng/index.php/njap/article/view/4885

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