Hepatoprotective Activity of Methanol Tuber Extract of Cyperus esculentus Linn on CCL4-Induced Hepatotoxicity in Albino Rats (Rattus norvegicus)

Authors

  • T.E. Ihedioha University of Nigeria, Nsukka, Enugu State, Nigeria
  • R. I. Odo Faculty of Veterinary Medicine, University of Nigeria, Nsukka, Enugu State, Nigeria.
  • C.E. Chiwetalu Faculty of Veterinary Medicine, University of Nigeria, Nsukka, Enugu State, Nigeria..
  • J. I. Ihedioha Faculty of Veterinary Medicine, University of Nigeria, Nsukka, Enugu State, Nigeria..

Keywords:

Hepatotoxicity, Cyperus esculentus, methanol tuber extract, albino rats, tiger nut

Abstract

This study investigated the hepatoprotective properties of methanol tuber extract of Cyperus esculentus on carbon tetrachloride (CCL4)-induced hepatotoxicity in albino rats. Fresh tubers of C. esculentus were collected, dried under shade, ground into powder, and extracted by cold maceration technique using 80% methanol. The extract obtained was filtered, dried, and subjected to phytochemical analysis. Thirty six (36) adult male albino rats were randomly assigned to 6 groups (A–F) of 6 rats each. Sub-acute hepatotoxicity was induced in groups A–E by intra-peritoneal (i/p) injection of 1 mg/kg body weight (bw) of CCL4 in equal volume of olive oil at 3 days interval for 12 days. Group A was given 10 ml/kg distilled water as placebo and served as untreated control, groups B, C, and D were treated with 200, 400 and 800 mg/kg Cyperus esculentus methanol tuber extract (CEME) respectively, group E was treated with 100 mg/kg silymarin as positive control, while group F was given 10 ml/kg distilled water as placebo and served as normal control. Treatment with CEME and silymarin was carried out for 15 days. Assay of serum enzymes and other bio-chemicals was done at the end of the 15-day experimental period following standard procedures. Treatment with CEME and silymarin significantly (p<0.05) reduced serum ALT, AST, ALP, bilirubin, and liver weight percentage of body weight, and also led to slight elevation of serum total protein of the albino rats whose liver were damaged using CCL4. These findings strongly suggest that CEME significantly protected the hepatocellular integrity, ameliorated impaired hepatic excretory function and slightly improved hepatic synthetic ability in experimentally induced sub-acute liver damage.

Author Biographies

T.E. Ihedioha, University of Nigeria, Nsukka, Enugu State, Nigeria

Departments of Veterinary Physiology and Pharmacology, Faculty of Veterinary Medicine, 

R. I. Odo, Faculty of Veterinary Medicine, University of Nigeria, Nsukka, Enugu State, Nigeria.

Departments of Veterinary Physiology and Pharmacology 

C.E. Chiwetalu, Faculty of Veterinary Medicine, University of Nigeria, Nsukka, Enugu State, Nigeria..

Departments of Veterinary Physiology and Pharmacology

J. I. Ihedioha, Faculty of Veterinary Medicine, University of Nigeria, Nsukka, Enugu State, Nigeria..

Veterinary Pathology and Microbiology

Downloads

Published

2024-07-09

How to Cite

Ihedioha, T. ., Odo, R. I. ., Chiwetalu, C. ., & Ihedioha, J. I. . (2024). Hepatoprotective Activity of Methanol Tuber Extract of Cyperus esculentus Linn on CCL4-Induced Hepatotoxicity in Albino Rats (Rattus norvegicus). Nigerian Journal of Animal Production, 977–980. Retrieved from https://njap.org.ng/index.php/njap/article/view/6139

Issue

Section

Articles

Most read articles by the same author(s)