Evaluation of Moringa oleifera, Psidium guajava Leaves Extracts and Oxytetracycline on Tissues Bacteriological Changes of Clarias gariepinus Juveniles

Abstract

The present study evaluated the effect of drumstick (Moringa oleifera) leaves and guava (Psidium guajava) leaves extracts and oxytetracycline on tissue bacteriological characteristics of Clarias gariepinus juveniles. Fish (8.90 ± 0.01g) were assigned to nine treatments with two replicates each. The treatment groups had different levels of M. oleifera and P. guajava leaves (1%, 2% and 3%) respectively and oxytetracycline (15 and 30mg/kg diet), the control group was without drumstick and guava leaves and oxytetracycline. Diet contained 40% crude protein, fed to the fish at 3% body weight twice daily for 56 days. Microbiological analyses of water and fish tissue (skin, gill, intestine and liver) were investigated using standard methods. Data were analyzed using ANOVA at p<0.05. The results of the study showed that all M. oleifera, P. guajava and oxytetracycline levels had decreased enterobacteriacea and total viable counts in water and fish tissues (skin, gill, intestine and liver) as inclusion rate increased and as months increased when compared to the control at 0, 4 and 8 weeks. It can be suggested that dietary supplementation of M. oleifera, P. guajava leaves extracts and oxytetracycline to fish diet can reduce bacterial growth and improve fish health.