β-Lactoglobulin Gene Polymorphism of Indigenous Nigerian Goat Breeds Using Polymerase Chain Reaction- Restriction Fragment Length Polymorphism

Auteur/ices

  • A. E Ezewudo Federal University of Technology, P.M.B 65, Minna, Niger State, Nigeria.
  • R. G. Abubakar Federal University of Technology, P.M.B 65, Minna, Niger State, Nigeria.
  • S. S. A. Egena Federal University of Technology, P.M.B 65, Minna, Niger State, Nigeria.
  • J. O. Alabi Federal University of Technology, P.M.B 65, Minna, Niger State, Nigeria.

DOI :

https://doi.org/10.51791/njap.vi.4074

Mots-clés :

polymorphism, goats, gene locus, PCR-RFLP, β-Lactoglobulin

Résumé

An investigation of the genetic polymorphism at the β-Lactoglobulin (β-LG) gene of Nigerian indigenous goat breeds (West
African Dwarf, Sahel and Red Sokoto) was carried out using Polymerase Chain Reaction-Restriction Fragment Length
Polymorphism (PCR-RFLP) method. Restriction endonucleases (Rsal) were used in the study to cut the extracted DNA into
fragments. The results provided evidence that the β-LG gene locus of West African Dwarf, Red Sokoto and Sahel goats is
monomorphic with only one genetic variant (allele A) with a gene frequency of 1.00. The amplified DNA products were observed
at 120 bp. The restriction digestion with sal revealed just one genotype at the β-LG locus. In conclusion, the results revealed the
absence of polymorphism at the β-LG ocus of the goats.

Biographies des auteurs

  • A. E Ezewudo, Federal University of Technology, P.M.B 65, Minna, Niger State, Nigeria.

    Department of Animal Production

  • R. G. Abubakar, Federal University of Technology, P.M.B 65, Minna, Niger State, Nigeria.

    Department of Animal Production

  • S. S. A. Egena, Federal University of Technology, P.M.B 65, Minna, Niger State, Nigeria.

    Department of Animal Production

  • J. O. Alabi, Federal University of Technology, P.M.B 65, Minna, Niger State, Nigeria.

    Department of Animal Production

Téléchargements

Publiée

2024-07-09

Numéro

2018: NSAP 2018 Proceedings

Rubrique

Articles

Comment citer

β-Lactoglobulin Gene Polymorphism of Indigenous Nigerian Goat Breeds Using Polymerase Chain Reaction- Restriction Fragment Length Polymorphism. (2024). Nigerian Journal of Animal Production, 7-9. https://doi.org/10.51791/njap.vi.4074