SELECTION OF STORAGE MEDIA FOR PIG A.I. — LABORATORY METHODS FOR REGULATING THE ACIDITY AND OSMOLARITY OF STORAGE MEDIA FOR BOAR SEMEN

Abstract

Simple laboratory methods were used to estimate optimal levels of acidity and osmolarity of four storage media for the boar semen. It was observed that above pH 7.0, less citric acid was needed for a unit change in pH of the sodium-citrate buffer, while above pH 8.0, more sodium carbonate and citric acid were needed for a unit change in the final Sodium-citrate buffered extender (Na-C), indicating high buffering capacity of this medium. The Glucose-glycine buffered extender (G-G), also required more sodium carbonate for a unit change of pH at above pH 6.5, also Indicating high buffering capacity of this medium. This was the same situation with the Tris-buffered extender (Tris), while the response with the Tissue - culture buffer extender (YCN) was always linear, indicating poor buffering capacity of the medium.