GENETIC DIVERSITY AT TLR7 GENE IN NIGERIAN INDIGENOUS CHICKENS AND ISA BROWN COMMERCIAL LAYER CHICKEN

Authors

  • H. O. Ukwu Federal University of Agriculture Makurdi, Benue State, Nigeria.
  • S. N. Ibe Michael Okpara University of Agriculture Umudike, Abia State, Nigeria.
  • E. N. Nwachukwu Michael Okpara University of Agriculture Umudike, Abia State, Nigeria.
  • I. G. Imumorin Georgia Institute of Technology, Atlanta, USA.
  • M. O. Audu Federal University of Agriculture Makurdi, Benue State, Nigeria.

DOI:

https://doi.org/10.51791/njap.vi.4409

Keywords:

Chicken, diversity, genetic distance, nucleotide, Toll-like receptor

Abstract

The objective of this study was to estimate genetic diversity at TLR7 locus in the Nigerian indigenous chicken populations and ISA Brown commercial layer chicken. Eight (8) chicken populations (naked neck, normal and frizzled-feathered Nigerian indigenous chickens in rain forest and Guinea Savannah regions respectively, Fulani ecotype chicken and ISA Brown commercial layer chicken) were used for this study. Blood samples were collected from each of the eight chicken populations. Genomic DNA was isolated from each blood sample using the Zymo Quick-gDNATM Miniprep kit. Sequencing of chTLR7 gene was done using the Sanger Sequencing Chemistry. Genetic distance estimates ranged from 0.007 to 0.054 and were close to zero, which suggests that the chickens have a close ancestor. The estimates of nearest-neighbour statistic ranged from 0.227 to 0.714, which showed that the chicken populations were part of the same panmictic population, hence were not genetically differentiated (P>0.05) at the TLR7 locus.

Author Biographies

H. O. Ukwu, Federal University of Agriculture Makurdi, Benue State, Nigeria.

Department of Animal Breeding and Physiology

S. N. Ibe, Michael Okpara University of Agriculture Umudike, Abia State, Nigeria.

Department of Animal Breeding and Physiology

E. N. Nwachukwu, Michael Okpara University of Agriculture Umudike, Abia State, Nigeria.

Department of Animal Breeding and Physiology

I. G. Imumorin, Georgia Institute of Technology, Atlanta, USA.

School of Biological Science

M. O. Audu, Federal University of Agriculture Makurdi, Benue State, Nigeria.

Department of Animal Breeding and Physiology

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Published

2024-07-07

How to Cite

Ukwu, H. O., Ibe, S. N., Nwachukwu, E. N., Imumorin, I. G., & Audu, M. O. (2024). GENETIC DIVERSITY AT TLR7 GENE IN NIGERIAN INDIGENOUS CHICKENS AND ISA BROWN COMMERCIAL LAYER CHICKEN. Nigerian Journal of Animal Production, 266–269. https://doi.org/10.51791/njap.vi.4409

Issue

2020: NSAP 2020 Proceedings

Section

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