EFFECT OF MORINGA OLEIFERA SAPONIN EXTRACT ON SERUM ENZYMES AND IMMUNOMODULATORY DOSES ADMINISTERED IN WISTAR RATS

Abstract

This study was aimed at investigating the impact of Moringa oleifera saponin extract on liver serum functions and immune response in Wistar rats. Wistar rats were assigned to seven dietary treatments of ten rats each in a completely randomised design consisting of a control (0.mg/L distil water) and three levels each of 1, 1.5 and 2mg/dL of both Moringa oleifera crude extracts and Moringa oleifera saponins respectively. The liver serum parameters were aspartate aminotransferase (AST), alanine aminotransferase (ALT), and alkaline phosphatase (ALP). Understanding changes in liver serum is essential for evaluating the potential hepatoprotective and immunomodulatory effects of Moringa oleifera saponin extract. There was no significant difference (P>0.05) for ALT and AST, together with Zinc and Immunoglobulin (IgD, IgE, IgG and IgM). While significant difference was observed for ALP, rats given 1 mg/mL moringa extract had the least mean value of ALP. There was Increase in the ALP value for rat given moringa extract from 1 mg/mL to 2 mg/mL while that of crude moringa saponins decreased in value. Values obtained for AST and ALT of rats administered crude moringa extract were apparently higher than those of rats administered moringa saponin and tannin extracts at similar doses. Immune response were observed between the means of all the treatment for Zn, IgD, IgE, IgG, IgM with control having significantly highest mean value compared to the treatments. With group given 1 mg/mL crude moringa Saponins having the least significant mean value. Therefore both moringa extract and crude moringa saponin increased in mean value for all parameters measured in the group administered 1 mg/mL to 2 mg/mL. Moreover, the phagocytic activity of immune cells, essential for clearing pathogens from the body, showed improvement in rats exposed to Moringa
oleifera saponin extract.