IN-VIVO TOXICITY EFFECT OF BIOCHEMICAL CONSTITUENT OF CLINACANTHUS NUTAN 80% METHANOL LEAF EXTRACT ON ZEBRAFISH EMBRYO

I. M.  Hassan; A. M.  Abdullahi; B.  Sa’idu; A.  Dahiru; N.  Abdulazeez; J. A.  Ishaq

doi:10.51791/njap.vi.6113

Authors

I. M. Hassan Usmanu Danfodiyo University Sokoto.
A. M. Abdullahi University of Maiduguri.
B. Sa’idu Usmanu Danfodiyo University Sokoto.
A. Dahiru Usmanu Danfodiyo University Sokoto.
N. Abdulazeez Usmanu Danfodiyo University Sokoto.
J. A. Ishaq Usmanu Danfodiyo University Sokoto.

DOI:

https://doi.org/10.51791/njap.vi.6113

Keywords:

Clinacanthus nutan, embryos, medicinal plants, toxicity, zebrafish

Abstract

Medicinal plants are used for therapeutic purposes against several diseases. Most of these plants contain high concentration of secondary metabolites that are of benefit to animals. When use in high concentration, plant extract can cause several side effects including malformation of tissue organs and mortality. Therefore, the aim of this research was to study the toxicity effect of Clinacanthus nutan 80% methanol leaf extract on zebrafish embryo. Leaf was extracted with 80% methanol, fertilized
Zebrafish embryos were exposed to the different concentration of the crude extract for 0-7 days. Results showed high yield of 30.12 %, there is significant difference (P<0.001) between the control and the treated groups. There was low toxicity with significant difference between the groups that were exposed to different concentrations and those that were maintained in embryo media. It was concluded that the low toxicity observed for this plant makes it beneficial as a medicinal supplement
that can be used in the prevention of diseases.