CRYOPRESERVATION OF SPERM CELLS OF LOCAL CHICKEN (GALLUS GALLUS DOMESTICUS) IN LIQUID NITROGEN

Authors

  • W. A. Olaniyi Adekunle Ajasin University, PMB 001, Akungba- Akoko, 342111, Ondo State, Nigeria
  • N. O. Bada Adekunle Ajasin University, PMB 001, Akungba- Akoko, 342111, Ondo State, Nigeria
  • A. Babatunde Adekunle Ajasin University, PMB 001, Akungba- Akoko, 342111, Ondo State, Nigeria
  • B. H. Ajao Obafemi Awolowo University, Ile- Tfe, 220005, Osun State, Nigeria
  • O. Bamidele Obafemi Awolowo University, Ile- Ife, 220005, Osun State, Nigeria
  • O. G. Omitogun Obafemi Awolowo University, Ile- Ife, 220005, Osun State, Nigeria

DOI:

https://doi.org/10.51791/njap.vi.6684

Keywords:

cryoprotectant, local chicken, sperm motility, sperm concentration

Abstract

This study evaluated the most effective cryoprotectant to be used for spermatozoa of local chicken Gallus
gallus domesticus in liquid nitrogen. Semen was collected from local chicken by means of abdominal massaging. The sperm motility and concentration of the. fresh, and liquid nitrogen cryopreserved semen were evaluated microscopically for maximum of three weeks. The cryoprotectant used were Dimethyl sulfoxide and Phosphate buffered saline (DP); Dimethyl sulfoxide and Ginzburg fish ringer (DF); Glycerol and Phosphate buffered saline (GP); Glycerol and Ginzburg fish ringer (GF); Egg yolk and Phosphate buffered saline (EY+P); Egg yolk and Ginzburg fish ringer (EY+F); Quail Egg yolk and Phosphate buffered saline (QEY+P); Quail Egg yolk and Ginzburg fish ringer (QEY+F). Results obtained showed that the motility and concentration of freshly collected sperm recorded 70% and 1.353 * I 0° spermatozoa/ml respectively, while there were decrease in sperm motility and concentration over time, but some cryoprotectants suchas QEY+P and EY+P performed best. Hence, these cryoprotectants are recommended for long term cryopreservation.

Author Biographies

W. A. Olaniyi, Adekunle Ajasin University, PMB 001, Akungba- Akoko, 342111, Ondo State, Nigeria

Department of Environmental Biology and Fisheries

N. O. Bada, Adekunle Ajasin University, PMB 001, Akungba- Akoko, 342111, Ondo State, Nigeria

Department of Environmental Biology and Fisheries

A. Babatunde, Adekunle Ajasin University, PMB 001, Akungba- Akoko, 342111, Ondo State, Nigeria

epartment of Environmental Biology and Fisheries

B. H. Ajao, Obafemi Awolowo University, Ile- Tfe, 220005, Osun State, Nigeria

Department of Animal Sciences

O. Bamidele, Obafemi Awolowo University, Ile- Ife, 220005, Osun State, Nigeria

Department of Animal Sciences

O. G. Omitogun, Obafemi Awolowo University, Ile- Ife, 220005, Osun State, Nigeria

Department of Animal Sciences

Downloads

Published

2024-07-15

How to Cite

Olaniyi, W. A., Bada, N. O., Babatunde, A., Ajao, B. H., Bamidele, O., & Omitogun, O. G. (2024). CRYOPRESERVATION OF SPERM CELLS OF LOCAL CHICKEN (GALLUS GALLUS DOMESTICUS) IN LIQUID NITROGEN. Nigerian Journal of Animal Production, 145–147. https://doi.org/10.51791/njap.vi.6684

Issue

2016: NSAP 2016 Proceedings

Section

Articles

Most read articles by the same author(s)

1 2 3 > >>