In-silico amplification of randomly selected male - specific region – Y (MSY) genes as candidate genes for the selection of fertile indigenous bulls for artificial insemination

Authors

  • A. B. Sikiru National Institute of Animal Nutrition and Physiology, India
  • S. S. A. Egena Federal University of Technology, Minna
  • O. M. Akinsola University of Jos, Nigeria
  • I. C. Alemede Federal University of Technology, Minna
  • J. O. Makinde Federal University, Gashua
  • S. O. Lawal University of Abuja

DOI:

https://doi.org/10.51791/njap.v46i5.286

Keywords:

Male-specific region-Y genes (MSY), molecular markers, bulls and in-silico

Abstract

Molecular markers-based selection is a novel tool for unbundling complexities associated with the selection of animals for breeding improvement programmes because these markers could contribute to full exploitation of animal potential prior to enrollment for improvement programmes. This study examined in-silico polymerase chain reaction amplification of some selected Male Specific region Y (MSY) genes in genome assemblies including Bos_taurus_UMD_3.1.1/bosTau8 and ARS-UCD1.2/bosTau9;BaylorBtau_4.6.1/bosTau7, Bos_taurus_UMD_3.1/bosTau6, and Baylor4.0/bosTau4. The results of the amplification showed that 12 out of the selected 30 genes were expressed at an average primer melting temperature of 61.78 oC; while salt and annealing oligo concentrations were 50 mM and 50 nM per reaction, respectively. Out of expressed genes; 6, 5 and 1 genes were expressed in genome assemblies Bos_taurus_UMD_3.1.1/bosTau8, ARS-UCD1.2/bosTau9, and BaylorBtau_4.6. 1/bosTau7, respectively. The study concluded that the expressed genes are potential markers for selection of fertile bulls and the primers used are suitable for in-vivo annotation of the genes in the prospective bull selection process for breeding programmes.

Author Biographies

A. B. Sikiru, National Institute of Animal Nutrition and Physiology, India

Reproductive Physiology Laboratory

S. S. A. Egena, Federal University of Technology, Minna

Department of Animal Production

O. M. Akinsola, University of Jos, Nigeria

Department of Veterinary Theriogenology and Production

I. C. Alemede, Federal University of Technology, Minna

Department of Animal Production

J. O. Makinde, Federal University, Gashua

Department of Animal Sciences

S. O. Lawal, University of Abuja

Department of Animal Science

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Published

2020-12-19

How to Cite

Sikiru, A. B., Egena, S. S. A., Akinsola, O. M., Alemede, I. C., Makinde, J. O., & Lawal, S. O. (2020). In-silico amplification of randomly selected male - specific region – Y (MSY) genes as candidate genes for the selection of fertile indigenous bulls for artificial insemination. Nigerian Journal of Animal Production, 46(5), 81 –. https://doi.org/10.51791/njap.v46i5.286

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