Organosomatic indices and tissues bacteriological changes of Clarias gariepinus On feeds supplemented with neem (Azadirachta indica) leaves and turmeric (Curcuma longa)rhizome extracts

Abstract

The study evaluated the effect of dietary doses of neem leaves and turmeric rhizome extracts on tissue bacteriology and organosomatic indices of Clarias gariepinus juveniles. Nine experimental diets containing control (0%),NL2 (1%),NL 3 (2%),NL 4 (3%), TR 5 (1%), TR 6 (2%), TR 7 (3%), CHRL 8 (15mg/kg diet) and CHRL 9 (30mg /kg diet) were formulated and replicated twice at 40% crude protein for 84 days. Microbial analyses of water, fish tissue and organ index of C. gariepinus were examined after 28, 56 and 84 days of feeding. Data were analyzed using ANOVA at p = 0.05. The results revealed that the fish fed treated experimental diets had decreased values of enterobacteriacea and total viable counts of bacterial loads from the water and fish tissues than the control at 28, 56 and 84 days. Turmeric rhizome extracts performed better when compared to the neem leaves and control. Also, organ index showed that liver, kidney and heart were not significantly increased in all the treated groups and the control. Neemleaves and turmeric rhizome extracts could be used in organic aquaculture to stimulate immunity, reduce and prevent bacterial infections in fish farming.