EVALUATION OF THE EFFECTS OF AMINO ACID SUBSTITUTION ON THE FUNCTION OF HEAT SHOCK PROTEIN 70 OF CHICKEN USING IN SILICO APPROACH

Abstract

The main objective of the study was to determine the effect of genetic variation resulting in amino acid substitution on the function of heat shock protein 70 of Chicken. Data on heat shock protein (HSP) 70 was retrieved from the database of National Centre for Biotechnology Information (NCBI). The 3D structure of HSP 70 was predicted using Phyre 2 server. Effect of amino acid substitution on the function of HSP was predicted using PANTHER server. MutPred 2 server was used to predict the mechanism of structural changes on HSP 70. The results indicated that all the amino acid substitutions evaluated had adverse effects and resulted in changes in the molecular structure of heat shock protein 70 of Chickens. It can be concluded that Single Nucleotide Polymorphism is a useful biomarker for evaluating the functions of heat shock protein 70 of Chickens.